A Secret Weapon For attune flow cytometer

A Secret Weapon For attune flow cytometer

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At that time, the early symptoms were being dismissed as debris, however the retrospective analysis demonstrated shared characteristics Along with the problematic cells in society. Tracing the infection assisted the lab set up added laboratory processes for screening and security of assay-significant cell strains.

As samples are acquired around the Attune CytPix Flow Cytometer, the higher-velocity brightfield digicam captures and retailers images of detected activities in a charge as many as six,000 images per second, based on the flow price and impression sizing. For better versatility, Attune Cytometric Software allows you to adjust the picture seize frequency as necessary and select a specific gate for graphic seize, if necessary.

Merge acoustic focusing technologies with significant-parameter Investigation for versatile spectral unmixing and standard workflows.

Find out new insights into your experimental details by visualizing populations applying combined fluorescence and morphology details. Our software package interface wizards permit downsampling, concatenation (merged documents) and dimensionality equipment that happen to be designed to the application for an intuitive, streamlined information analysis practical experience.

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Listed here, a qualified user has gated singlets confidently. Right after analyzing the manual singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate consists of much more than four% aggregates.

Optimizing gating technique. Even sturdy manual singlet gating is mistake-susceptible and remains a subjective decision level in Just about all flow cytometry assays. Imaging may be used to substantiate and adjust gates to incorporate only solitary cells of desire.

Regular image high quality even at higher throughput Acoustic focusing along with a substantial-pace digital camera Incorporate to graphic these T cells continuously at reduced or superior flow charges. Very easily change concentrate and digicam options to fulfill experimental requirements.

Early signs of this contamination had at first been dismissed as particles. Backgating to find new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also allows you to use morphological features to find appealing subpopulations that might not be obvious from flow cytometry details on your own.

Customers can check out party images in authentic-time to substantiate sample high-quality, enhance protocols, and report the morphology of each and every cell inhabitants determined for analysis. Imaging capture also Positive aspects from acoustic concentrating, which helps you to placement the cells to ensure that a pointy, centered impression is acquired.

AI-driven automated graphic analysis With all the Attune Cytometric Computer software translates celebration capabilities into distinct morphology parameters which might be combined with normal fluorescence and scatter parameters to reveal extremely reproducible populace improvements and distinctive subsets.

We previously demonstrated the power of imaging Automobile-T/Ramos cell interactions. Let’s have a here look at just the population of biggest curiosity, the double positive functions, To find out more. We will now use prolonged morphological parameters (below, circularity and skewness of depth) to further take a look at the features of these populations and refine gating on these functions, growing information robustness.

Leverage the strength of Innovative technologies and innovation to accelerate your discoveries. Belief inside the Attune Flow Cytometer loved ones to deliver the reliability, speed, and precision that set your work apart.

No wash/no lyse leukocyte Evaluation with imaging. Cells had been obtained from 24-hour-previous blood by dilution in one mM EDTA ( Pro UX/UI allows customers to coach AI models for expanded purposes. (A) The “Coach Design” characteristic supplies annotation instruments and serious-time design coaching accuracy.

Gating on these prolonged parameters means that you can promptly and correctly detect populations of interest and make sure gating strategy with little or no handbook evaluation. The Attune software offers access to state-of-the-art device Understanding Assessment tools inside of an expertly designed person interface, rendering it exceptionally user friendly.

As the cell line had Earlier been analyzed about the Attune CytPix Flow Cytometer, the scientists went back again to the photographs and were capable of doc the microbial infection at the very least five times previously.

The Attune CytPix Flow Cytometer makes it possible for end users to visualise samples for the duration of acquisition, verify the precision of gating tactics, and assistance success with trusted documentation for publication functions.

What seemed to be a homogeneous populace is definitely a lot more diverse—an insight that should be regarded as when interpreting final results.